Hi Mariano,

Thanks for your email. There was a permission issue with the cache that should 
now be resolved as I was able to manually build your package. Let's see how it 
does next week.

Andres Wokaty (they/them)

Waldron Lab at CUNY SPH
Bioconductor Core Team
________________________________
From: Bioc-devel <[email protected]> on behalf of Mariano Ruz 
Jurado <[email protected]>
Sent: Thursday, October 23, 2025 5:03 AM
To: [email protected] <[email protected]>
Subject: [Bioc-devel] DOtools vignette build errors due to "Permission Denied"

* This email originates from a sender outside of CUNY. Verify the sender before 
replying or clicking on links and attachments. *

Hello,
I am the maintainer of the DOtools package and I got an error in the vignette 
build that I cannot reproduce, since it seems to be a permission problem.
When I look at the build report 
(https://urldefense.com/v3/__https://bioconductor.org/checkResults/3.22/bioc-LATEST/DOtools/nebbiolo2-buildsrc.html__;!!LRXxDv2l!VQFvpsmo0moRYvQVw1X9qBWN5d8P0So9mnh01xkOuWfPUinbSCXrq-26TStQ8BhD8kL3pG7d_TNz5qq-tdtbuKOZB6dsTzOVI38UtnLc$
 )
I see the following error:

Quitting from DOtools.Rmd:279-286 [annotation]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
<error/rlang_error>
Error in `lock()`:
! Cannot open lock file: Permission denied
---
Backtrace:
�g
1. �|�wDOtools::DO.CellTypist(...)
2. �|�wbasilisk::basiliskRun(...)
3. �|�wbasilisk::basiliskStart(...)
4. �|�wbasilisk::obtainEnvironmentPath(env)
5. �|�wdir.expiry::lockDirectory(envdir, exclusive = !file.exists(envpath))
6. �|�wfilelock::lock(plock, exclusive = FALSE)
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

It seems to be a problem related to running python code in a basilisk 
environment.
Some functions of the DOtools rely heavily on python code and therefore the 
vignette build seems to fail whenever I call such a function.
Is there a nice way of handling this problem?

Best regards and many thanks,
Mariano


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