chrM is the broken old hg19 mitogenome

chrMT is rCRS, same as hg38 chrM

--t

> On Oct 12, 2025, at 12:30 PM, Raymond Cavalcante <[email protected]> wrote:
> 
> Hi,
> 
> I was testing the annotatr package after fixing the ggplot2 v4 stuff, but I
> got an error in my vignette build and discovered what I think is an error
> in the result of Seqinfo::seqinfo(genome = 'hg19').
> 
> There are two mitochondrial chromosomes for hg19. This causes some
> downstream issues when merging hg19 GRanges.
> 
>> Seqinfo::Seqinfo(genome='hg19')[c('chrM', 'chrMT')]
> Seqinfo object with 2 sequences (2 circular) from hg19 genome:
>  seqnames seqlengths isCircular genome
>  chrM          16571       TRUE   hg19
>  chrMT         16569       TRUE   hg19
> 
> I checked hg38 on a lark and it appears correct.
> 
>> Seqinfo::Seqinfo(genome='hg38')[c('chrM', 'chrMT')]
> Seqinfo object with 2 sequences (1 circular) from 2 genomes (hg38, NA):
>  seqnames seqlengths isCircular genome
>  chrM          16569       TRUE   hg38
>  chrMT            NA         NA   <NA>
> 
> I'm using 0.99.2 of Seqinfo in a Bioconductor devel Docker image.
> 
> My session info:
> 
>> devtools::session_info()
> ─ Session info
> ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
> setting  value
> version  R version 4.5.1 (2025-06-13)
> os       Ubuntu 24.04.3 LTS
> system   x86_64, linux-gnu
> ui       X11
> language (EN)
> collate  en_US.UTF-8
> ctype    en_US.UTF-8
> tz       Etc/UTC
> date     2025-10-12
> pandoc   3.8.1 @ /usr/bin/pandoc
> quarto   1.7.32 @ /usr/local/bin/quarto
> 
> ─ Packages
> ───────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
> !  package                           * version   date (UTC) lib source
>    abind                               1.4-8     2024-09-12 [1] RSPM (R
> 4.5.0)
>    AnnotationDbi                     * 1.71.1    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    AnnotationHub                       3.99.6    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
> VP annotatr                          * 1.35.1    2025-05-13 [?]
> Bioconductor 3.22 (R 4.5.0) (on disk 1.35.0)
>    Biobase                           * 2.69.1    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    BiocFileCache                       2.99.6    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    BiocGenerics                      * 0.55.3    2025-10-09 [1]
> Bioconductor 3.22 (R 4.5.1)
>    BiocIO                              1.19.0    2025-04-15 [1]
> Bioconductor 3.22 (R 4.5.0)
>    BiocManager                         1.30.26   2025-06-05 [1] CRAN (R
> 4.5.1)
>    BiocParallel                        1.43.4    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    BiocVersion                         3.22.0    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    Biostrings                          2.77.2    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    bit                                 4.6.0     2025-03-06 [1] RSPM (R
> 4.5.0)
>    bit64                               4.6.0-1   2025-01-16 [1] RSPM (R
> 4.5.0)
>    bitops                              1.0-9     2024-10-03 [1] RSPM (R
> 4.5.0)
>    blob                                1.2.4     2023-03-17 [1] RSPM (R
> 4.5.0)
>    brio                                1.1.5     2024-04-24 [1] RSPM (R
> 4.5.0)
>    BSgenome                            1.77.2    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    cachem                              1.1.0     2024-05-16 [1] RSPM (R
> 4.5.0)
>    cli                                 3.6.5     2025-04-23 [1] RSPM (R
> 4.5.0)
>    codetools                           0.2-20    2024-03-31 [2] CRAN (R
> 4.5.1)
>    crayon                              1.5.3     2024-06-20 [1] RSPM (R
> 4.5.0)
>    curl                                7.0.0     2025-08-19 [1] RSPM (R
> 4.5.0)
>    DBI                                 1.2.3     2024-06-02 [1] RSPM (R
> 4.5.0)
>    dbplyr                              2.5.1     2025-09-10 [1] RSPM (R
> 4.5.0)
>    DelayedArray                        0.35.3    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    desc                                1.4.3     2023-12-10 [1] RSPM (R
> 4.5.0)
>    devtools                            2.4.6     2025-10-03 [1] RSPM (R
> 4.5.0)
>    dplyr                               1.1.4     2023-11-17 [1] RSPM (R
> 4.5.0)
>    ellipsis                            0.3.2     2021-04-29 [1] RSPM (R
> 4.5.0)
>    farver                              2.1.2     2024-05-13 [1] RSPM (R
> 4.5.0)
>    fastmap                             1.2.0     2024-05-15 [1] RSPM (R
> 4.5.0)
>    filelock                            1.0.3     2023-12-11 [1] RSPM (R
> 4.5.0)
>    fs                                  1.6.6     2025-04-12 [1] RSPM (R
> 4.5.0)
>    generics                          * 0.1.4     2025-05-09 [1] RSPM (R
> 4.5.0)
>    GenomeInfoDb                        1.45.12   2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    GenomicAlignments                   1.45.4    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    GenomicFeatures                   * 1.61.6    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    GenomicRanges                     * 1.61.5    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    ggplot2                             4.0.0     2025-09-11 [1] RSPM (R
> 4.5.0)
>    glue                                1.8.0     2024-09-30 [1] RSPM (R
> 4.5.0)
>    gtable                              0.3.6     2024-10-25 [1] RSPM (R
> 4.5.0)
>    hms                                 1.1.3     2023-03-21 [1] RSPM (R
> 4.5.0)
>    httr                                1.4.7     2023-08-15 [1] RSPM (R
> 4.5.0)
>    httr2                               1.2.1     2025-07-22 [1] RSPM (R
> 4.5.0)
>    IRanges                           * 2.43.5    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    jsonlite                            2.0.0     2025-03-27 [1] RSPM (R
> 4.5.0)
>    KEGGREST                            1.49.2    2025-10-10 [1]
> Bioconductor 3.22 (R 4.5.1)
>    lattice                             0.22-7    2025-04-02 [2] CRAN (R
> 4.5.1)
>    lifecycle                           1.0.4     2023-11-07 [1] RSPM (R
> 4.5.0)
>    magrittr                            2.0.4     2025-09-12 [1] RSPM (R
> 4.5.0)
>    Matrix                              1.7-4     2025-08-28 [1] RSPM (R
> 4.5.0)
>    MatrixGenerics                      1.21.0    2025-04-15 [1]
> Bioconductor 3.22 (R 4.5.0)
>    matrixStats                         1.5.0     2025-01-07 [1] RSPM (R
> 4.5.0)
>    memoise                             2.0.1     2021-11-26 [1] RSPM (R
> 4.5.0)
>    org.Hs.eg.db                      * 3.22.0    2025-10-11 [1]
> Bioconductor
>    pillar                              1.11.1    2025-09-17 [1] RSPM (R
> 4.5.0)
>    pkgbuild                            1.4.8     2025-05-26 [1] RSPM (R
> 4.5.0)
>    pkgconfig                           2.0.3     2019-09-22 [1] RSPM (R
> 4.5.0)
>    pkgload                             1.4.1     2025-09-23 [1] RSPM (R
> 4.5.0)
>    plyr                                1.8.9     2023-10-02 [1] RSPM (R
> 4.5.0)
>    png                                 0.1-8     2022-11-29 [1] RSPM (R
> 4.5.0)
>    purrr                               1.1.0     2025-07-10 [1] RSPM (R
> 4.5.0)
>    R6                                  2.6.1     2025-02-15 [1] RSPM (R
> 4.5.0)
>    rappdirs                            0.3.3     2021-01-31 [1] RSPM (R
> 4.5.0)
>    RColorBrewer                        1.1-3     2022-04-03 [1] RSPM (R
> 4.5.0)
>    Rcpp                                1.1.0     2025-07-02 [1] RSPM (R
> 4.5.0)
>    RCurl                               1.98-1.17 2025-03-22 [1] RSPM (R
> 4.5.0)
>    readr                               2.1.5     2024-01-10 [1] RSPM (R
> 4.5.0)
>    regioneR                            1.41.3    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    remotes                             2.5.0     2024-03-17 [1] RSPM (R
> 4.5.0)
>    reshape2                            1.4.4     2020-04-09 [1] RSPM (R
> 4.5.0)
>    restfulr                            0.0.16    2025-06-27 [1] RSPM (R
> 4.5.1)
>    rjson                               0.2.23    2024-09-16 [1] RSPM (R
> 4.5.0)
>    rlang                               1.1.6     2025-04-11 [1] RSPM (R
> 4.5.0)
>    rprojroot                           2.1.1     2025-08-26 [1] RSPM (R
> 4.5.0)
>    Rsamtools                           2.25.3    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    RSQLite                             2.4.3     2025-08-20 [1] RSPM (R
> 4.5.0)
>    rstudioapi                          0.17.1    2024-10-22 [1] RSPM (R
> 4.5.0)
>    rtracklayer                       * 1.69.1    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    S4Arrays                            1.9.1     2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    S4Vectors                         * 0.47.4    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    S7                                  0.2.0     2024-11-07 [1] RSPM (R
> 4.5.0)
>    scales                              1.4.0     2025-04-24 [1] RSPM (R
> 4.5.0)
>    Seqinfo                           * 0.99.2    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    sessioninfo                         1.2.3     2025-02-05 [1] RSPM (R
> 4.5.0)
>    SparseArray                         1.9.1     2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    stringi                             1.8.7     2025-03-27 [1] RSPM (R
> 4.5.0)
>    stringr                             1.5.2     2025-09-08 [1] RSPM (R
> 4.5.0)
>    SummarizedExperiment                1.39.2    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    testthat                          * 3.2.3     2025-01-13 [1] RSPM (R
> 4.5.0)
>    tibble                              3.3.0     2025-06-08 [1] RSPM (R
> 4.5.0)
>    tidyselect                          1.2.1     2024-03-11 [1] RSPM (R
> 4.5.0)
>    TxDb.Hsapiens.UCSC.hg19.knownGene * 3.22.0    2025-10-11 [1]
> Bioconductor
>    tzdb                                0.5.0     2025-03-15 [1] RSPM (R
> 4.5.0)
>    UCSC.utils                          1.5.0     2025-04-17 [1]
> Bioconductor 3.22 (R 4.5.0)
>    usethis                             3.2.1     2025-09-06 [1] RSPM (R
> 4.5.0)
>    vctrs                               0.6.5     2023-12-01 [1] RSPM (R
> 4.5.0)
>    withr                               3.0.2     2024-10-28 [1] RSPM (R
> 4.5.0)
>    XML                                 3.99-0.19 2025-08-22 [1] RSPM (R
> 4.5.0)
>    XVector                             0.49.1    2025-10-07 [1]
> Bioconductor 3.22 (R 4.5.1)
>    yaml                                2.3.10    2024-07-26 [1] RSPM (R
> 4.5.0)
> 
> Thanks!
> 
> --------------------
> Raymond Cavalcante, PhD
> Bioinformatics Analyst
> Epigenomics Core and Bioinformatics Core
> Biomedical Research Core Facilities
> University of Michigan Medical School
> 
> [email protected]
> [email protected]
> 
>    [[alternative HTML version deleted]]
> 
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