Dear all,
I’m encountering the following error when loading the pbmc_multimodal.h5seurat 
file in RStudio. All required libraries are installed and loaded:
The output proceeds as expected until the following error occurs and the 
reference won't load. I tried to load without image and with specific assay too 
but nothing works:"assay = "SCT", assay.images = FALSE"

> library(Seurat)
> library(SeuratDisk)
> library(rhdf5)> reference <- 
> LoadH5Seurat("/home/local/BICR/hjabeen/QC/Results/pbmc_multimodal.h5seurat")Validating
>  h5Seurat fileInitializing ADT with dataAdding counts for ADTAdding variable 
> feature information for ADTAdding miscellaneous information for 
> ADTInitializing SCT with dataAdding counts for SCTAdding variable feature 
> information for SCTAdding miscellaneous information for SCTAdding reduction 
> apcaAdding cell embeddings for apcaAdding feature loadings for apcaAdding 
> miscellaneous information for apcaAdding reduction aumapAdding cell 
> embeddings for aumapAdding miscellaneous information for aumapAdding 
> reduction pcaAdding cell embeddings for pcaAdding feature loadings for 
> pcaAdding miscellaneous information for pcaAdding reduction spcaAdding cell 
> embeddings for spcaAdding feature loadings for spcaAdding miscellaneous 
> information for spcaAdding reduction umapAdding cell embeddings for 
> umapAdding miscellaneous information for umapAdding reduction wnn.umapAdding 
> cell embeddings for wnn.umapAdding miscellaneous information for 
> wnn.umapAdding graph wknnAdding graph wsnnError in unlist(x = assays.images, 
> index$global$images) : 'recursive' must be a length-1 vector
 
I would greatly appreciate any suggestions.
Many thanks in advance for your help!
Best regards,HiraSchool of Cancer SciencesUniversity of Glasgow, Scotland




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