Hi, Hervé.
Thank you for making this clear to me. I will try to think of an optimal solution for this. The issue here is that my package works as the pipeline itself, similarly to how ORFik works. Out of curiosity, I just checked how ORFik and KnowSeq handle this situation: * for STAR, for instance, ORFik simply comments the function that runs STAR in @examples (https://github.com/Roleren/ORFik/blob/master/R/STAR.R). Quite a hacky solution to avoid the overuse of \donttest{}. * KnowSeq includes a function to download all external software (https://github.com/CasedUgr/KnowSeq/blob/75d5d9f526f5b4ac561455a46884fe0a1860ffa0/R/sraToFastq.R), and it includes \donttest{} in some functions. I will see if I can include \donttest{} in as many functions with external dependencies as I can and add some other dependencies in SystemRequirements to satisfy the 80% testable code in @examples. Best, ========================= Fabrício de Almeida Silva Undergraduate degree in Biological Sciences (UENF) MSc. candidate in Plant Biotechnology (PGBV/UENF - RJ/Brazil) Laboratório de Química e Função de Proteínas e Peptídeos (LQFPP/CBB/UENF - RJ/Brazil) Personal website: https://almeidasilvaf.github.io ________________________________ De: Hervé Pagès <hpages.on.git...@gmail.com> Enviado: segunda-feira, 23 de agosto de 2021 16:57 Para: Fabricio de Almeida <fabricio_almeidasi...@hotmail.com>; bioc-devel@r-project.org <bioc-devel@r-project.org> Assunto: Re: [Bioc-devel] External dependencies and reproducibility in all platforms Hi Fabricio, If your package requires external software/libraries/tools in order to pass 'R CMD build' and 'R CMD check', then please list them in the SystemRequirements field of your DESCRIPTION file. In addition, we kindly ask you to provide an INSTALL file in the top-level folder of your package source tree that documents how to install these external deps on all the supported platforms. BTW I'm not sure that KnowSeq or ORFik have external system requirements. I don't see that they have a SystemRequirements field. Only openPrimeR has one but it's not clear to me that the package actually needs all the things listed there e.g. for example MAFFT is listed but we don't have it on the build machines. FWIW most packages avoid having to depend on external tools like SRAtoolkit, STAR or salmon by assuming that this step of the analysis was already taken care of, and by focusing on the downstream analysis. These packages often include the output of the upstream analysis as a small dataset and start from there. Hope this helps, Best, H. On 23/08/2021 07:10, Fabricio de Almeida wrote: > Dear Bioc developers, > > I am writing a package that contains external dependencies, and I'd like to > know what are the best practices to submit this kind of package to > Bioconductor. > > The external dependencies are standard RNA-seq analysis algorithms, such as > SRAtoolkit, STAR and salmon. I have seen other Bioc packages with external > dependencies, such as KnowSeq > (https://bioconductor.org/packages/release/bioc/html/KnowSeq.html), ORFik > (https://www.bioconductor.org/packages/release/bioc/html/ORFik.html), and > openPrimeR > (https://bioconductor.org/packages/release/bioc/html/openPrimeR.html), but it > is not clear how they handle the dependencies in the Bioconductor build > system. > > I have a conda environment containing all the dependencies + R 4.1.0, which > works fine. However, conda is not the best option, as some dependencies may > not exist in all OS, particularly in Windows. > > Perhaps a Docker container with the dependencies in an Ubuntu OS would ensure > reproducibility in all platforms, but what should I do for the package to > pass all checks in the Bioc build system? > > Any help is appreciated. > > Best, > > > ========================= > > > Fabr�cio de Almeida Silva > > Undergraduate degree in Biological Sciences (UENF) > > MSc. candidate in Plant Biotechnology (PGBV/UENF - RJ/Brazil) > > Laborat�rio de Qu�mica e Fun��o de Prote�nas e Pept�deos (LQFPP/CBB/UENF - > RJ/Brazil) > > Personal website: https://almeidasilvaf.github.io > > > [[alternative HTML version deleted]] > > > _______________________________________________ > Bioc-devel@r-project.org mailing list > https://stat.ethz.ch/mailman/listinfo/bioc-devel > -- Hervé Pagès Bioconductor Core Team hpages.on.git...@gmail.com [[alternative HTML version deleted]] _______________________________________________ Bioc-devel@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/bioc-devel
