Hello.

I have a question regarding how to set qnameSuffixStart and qnamePrefixEnd to 
be able to read paired reads from bam files:


Many .bam files I worked with have qnames in the format SAMPLENAME.READID.1 and 
SAMPLENAME.READID.2 .


I was wondering how I can set qnameSuffixStart and qnamePrefixEnd params in 
BamFile function of Rsamtools to be able to make functions such as 
readGAlignmentPairs() of GenomicAlignments properly distinguish paired reads 
based on the READID.


Would setting qnameSuffixStart="." and qnamePrefixEnd="." do the trick?


and What happens if I only set qnamePrefixEnd="." ? Is it greedy? Does it 
neglect everything after the first "." in qname (i.e. considers SAMPLENAME) or 
does it omit everything after the last "."  (i.e. considers SAMPLENAME.READID)?


Cheers,


Ali


--

Ali Oghabian
Bioinformatics,

RNA-splicing laboratory (Room 4024B),

Institute of Biotechnology,
P.O.Box 56 (Viikinkaari 5),
00014 University of Helsinki
Finland.

Email: 
ali.oghab...@helsinki.fi<https://webmail.helsinki.fi/horde/imp/dynamic.php?page=mailbox#>
Phone: +358 50 4484569

        [[alternative HTML version deleted]]

_______________________________________________
Bioc-devel@r-project.org mailing list
https://stat.ethz.ch/mailman/listinfo/bioc-devel

Reply via email to