Hi Julian,

At the root of the problem is what rbind() does on DataFrames containing
matrices:

  m <- matrix(1:4, nrow=2)
  df <- DataFrame(m=I(m))
  df2 <- rbind(df, df)

Then:

  > df2
  DataFrame with 8 rows and 1 column
           m
    <matrix>
  1      1 3
  2      2 4
  3      1 3
  4      2 4

  > nrow(df2)
  [1] 8

Too many rows!

  > str(df2)
  Formal class 'DataFrame' [package "IRanges"] with 6 slots
    ..@ rownames       : NULL
    ..@ nrows          : int 12
    ..@ listData       :List of 1
    .. ..$ m: int [1:6, 1:2] 1 2 3 1 2 3 4 5 6 4 ...
    ..@ elementType    : chr "ANY"
    ..@ elementMetadata: NULL
    ..@ metadata       : list()

  > validObject(df2)
  [1] TRUE

I'll leave this to Michael.

Thanks,
H.


On 05/20/2014 01:22 AM, Julian Gehring wrote:
Hi,

If I want to bind two GRanges object with a matrix in the meta columns,
the concatenation of the two fails in bioc-stable (GenomicRanges 1.16.3)
and bioc-devel (GenomicRanges 1.17.13) with:

'''
Error in validObject(.Object) :
   invalid class “GRanges” object: number of rows in DataTable
'mcols(x)' must match length of 'x'
'''

If multiple columns are used, the class of of the first column seem to
determine the behavior:

#+BEGIN_SRC R
   library(GenomicRanges)

   ## sample data, two identical GRanges
   gr1 = gr2 = GRanges(1, IRanges(1:2, width = 1))
   m = matrix(1:4, 2)

   ## the vector alone works
   mcols(gr1) = mcols(gr2) = DataFrame(x = 1)
   c(gr1, gr2) ## works

   ## vector first, matrix second works
   mcols(gr1) = mcols(gr2) = DataFrame(x = 1, m = I(m))
   c(gr1, gr2) ## works

   ## the matrix alone fails
   mcols(gr1) = mcols(gr2) = DataFrame(m = I(m))
   c(gr1, gr2) ## fails

   ## matrix first, vector second fails
   mcols(gr1) = mcols(gr2) = DataFrame(m = I(m), x = 1)
   c(gr1, gr2) ## fails
#+END_SRC

Best wishes
Julian

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Hervé Pagès

Program in Computational Biology
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