How about providing the code that you are working with?

This ought to work:
s/C:\\Dir 1\\Dir 2\\Dir with more spaces/replacement/

__CODE__
$in = 'C:\Dir 1\Dir 2\Dir with more spaces';
$in =~ s/C:\\Dir 1\\Dir 2\\Dir with more spaces/replacement/;
print "$in\n";
__OUTPUT__
replacement

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