hellow: I have written a script, but it does not work, can you tell me what wrong with me?
#! /usr/bin/perl use strict; use warnings; use Getopt::Long; my ($dir, $files, $file_name, $file_format, $file_dir, $file_main); GetOptions ('dr=s' =>\$dir); open INF,"<",'sine.fa' or die "can't read open sine"; foreach $files (glob("$dir/*.fa")) { open FASEQ, "<", $files or die "can not read open $!"; ($file_dir, $file_main) = split (/\/\//,$files); ($file_name,$file_format) =split(/\./,$file_main); open OUTFILE, '>', $file_name.".txt"; `./usearch -ublast FASEQ -db INF -evalue 1e-5 -userout OUTFILE -strand both -userfields query+qlo+qhi+ql+qs+qstrand+target+tlo+thi+tl`; } close FASEQ; close OUTFILE; close INF; when I run the script, and the results is "FASEQ can not open". additional, the results from glob as follows: ./fadata//carp-carp01_TKD181002053-1_1.fa ./fadata//carp-carp01_TKD181002053-1_2.fa ...... -- 湖南师范大学生命科学院:刘 东 格言:积土而为山,积水而为海; 知识在于积累,成功在于勤奋。