Check out win32com (I am assuming you want to do this from a Windows machine). Here is a small snippet on reading the value from cell A1 in the active sheet. from win32com.client import Dispatch flxls = "c:\\my\\file.xls" xl = Dispatch ("Excel.Application") xl.Visible = True xl.Workbooks.Open(flxls) workbook = xl.ActiveWorkbook sheet = xl.ActiveSheet param_1 = sheet.Cells(1,1).Value workbook.Close() xl.Quit()
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